Advanced Methods in Molecular Biology and Biotechnology
A Practical Lab Manual
- 1 Edición - 28 de octubre de 2020
- Última edición
- Autores: Khalid Z. Masoodi, Sameena Maqbool Lone, Rovidha Saba Rasool
- Idioma: Inglés
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biote… Leer más
Descripción
Descripción
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.
This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.
Puntos claves
Puntos claves
- Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology
- Features clear, step-by-step instruction for applying the techniques covered
- Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
De interès para
De interès para
Undergraduate and graduate students of molecular biology and / or biotechnology
Índice
Índice
1. Introduction to molecular biology techniques2. Agarose gel electrophoresis3. SDS-polyacrylamide gel electrophoresis4. Immunoelectrophoresis5. DNA plasmid isolation using Alkaline Lysis Method from E. coli DH5α cells6. Restriction digestion of Plasmid DNA7. Genomic DNA extraction from plant leaves using CTAB method8. Genomic DNA extraction from silkworm using modified phenol: chloroform method (Suzuki et al., 1972)9. Genomic DNA extraction from Fungal Mycelium using modified Cenis, 1992 method10. Bacterial transformation protocol using CaCl2
11. Transformation of E. coli by electroporation12. Transfection of cells using lipofectamine13. Total RNA isolation14. RNA isolation from plant tissues with high phenolic compounds and polysaccharides15. Total protein extraction with TCA-acetone and 2D gel electrophoresis16. Dansyl-Edman method of peptide sequencing17. ISSR analysis18. Gene cloning/recombinant DNA technology19. Polymerase Chain Reaction (PCR)20. Blotting/ hybridization and its types21. Dot blot analysis22. ELISA (enzyme-linked immunosorbent assay)23. Study of principle of centrifugation
24. Study of principle of chromatography
25. Thin layer chromatography (TLC)
26. Gel-filtration or size-exclusion chromatography
27. Ion-exchange chromatography
28. To perform affinity chromatography
29. To perform paper chromatography
30. Growth of bacterial cultures and preparation of growth curve
31. To study standard operating procedures (SOPs) for various equipment
Detalles del producto
Detalles del producto
- Edición: 1
- Última edición
- Publicado: 4 de noviembre de 2020
- Idioma: Inglés
Sobre los autores
Sobre los autores
KM
Khalid Z. Masoodi
Khalid Z. Masoodi is an Associate Professor and Senior Scientist in the Division of Plant Biotechnology at Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India. He is the recipient of a number of awards and holds numerous patents. He has two decades worth of teaching and research experience in the areas of plant biotechnology, molecular biology, cancer research, and human disease research, and has published numerous articles and books. He leads a transcriptomics laboratory and works extensively with fluorescence imaging, real-time PCR, cell culture, and Western blotting platforms. His scientific interests include transcription biology in plant stress responses and cancer, as well as the discovery of new drugs from medicinal plants.
SL
Sameena Maqbool Lone
Sameena Maqbool Lone is a biotechnology researcher with a strong focus on vegetable science, molecular biotechnology, and sustainable bioproduction. She is currently a postdoctoral researcher in the Division of Vegetable Science, Sher-e- Kashmir University of Agricultural Sciences and Technology of Kashmir, India. Her current work explores the modulation of inflammatory and oncogenic signalling pathways, positioning plant-derived compounds within the broader framework of translational cancer therapeutics. Her research interests include phytochemical science, molecular diversity analysis, and the application of plant resources for health-related innovation. She holds a number of patents and has co-authored several research papers and books.
RR