Advances in Virus Research

Chapter One: Functional Genomic Strategies for Elucidating Human–Virus Interactions: Will CRISPR Knockout RNAi and Haploid Cells?

• Abstract
• 1 Introduction
• 2 Host–Virus Genetic Screens
• 3 RNAi Genetic Screening Technologies and Approaches
• 4 Haploid Cell Genetic Screening Technology and Approach
• 5 CRISPR/Cas9 Genetic Screening Technologies and Approaches
• 6 Comparison of HRV-HF Screens: Arrayed MORR RNAi Versus Pooled CRISPR/Cas9
• 7 Future Directions
• Acknowledgments

Chapter Two: Alphaherpesvirus Latency: A Dynamic State of Transcription and Reactivation

• Abstract
• 1 Introduction
• 2 A Working Definition of Latency
• 3 Model Systems to Study Alphaherpesvirus Latency
• 4 Establishment of Latency
• 5 The Dynamic State of Alphaherpesvirus Latency
• 6 Conclusions and Unanswered Questions

Chapter Three: Nuclear Egress of Herpesviruses: The Prototypic Vesicular Nucleocytoplasmic Transport

• Abstract
• 1 Introduction
• 2 The Nucleus
• 3 Herpesvirus Nuclear Egress
• 4 Nuclear Egress as a Cellular Nuclear Export Mechanism
• 5 Alternative Herpesvirus Nuclear Escape Pathways
• 6 Nuclear Egress in Other Virus Families
• 7 Summary
• Acknowledgments

Chapter Four: Structure and Associated Biological Functions of Viroids

• Abstract
• 1 Introduction
• 2 Biophysical Properties as Basis for Functional Activity
• 3 Structure of Pospiviroidae
• 4 Structure of Avsunviroidae
• 5 Structural Motifs and Their Biological Function
• 6 Perspective
• Acknowledgments

Index