Arginase: Structure, Function, and Therapeutic Targeting
- 1 Edición, Volumen 740 - 15 de mayo de 2026
- Última edición
- Editor: Luigi Di Costanzo
- Idioma: Inglés
Arginase: Structure, Function, and Therapeutic Targeting, Volume 740 in the Methods in Enzymology series, provides a comprehensive, methodology-driven perspective on arginase rese… Leer más
Descripción
Descripción
Additional chapters cover Dual ARG1/ARG2 Inhibition: Experimental and Structural Strategies for Inhibitor Design and Optimization, Synthesis of Second-Generation Arginase Inhibitors, Cryo-EM Workflow and Structural Characterization of Arginase–Antibody Complexes, Development and Validation of an Electrochemical Assay for Arginase Activity, Experimental and Structural Analysis of H. pylori Arginase in Gastric Immune Evasion, Agmatinase and Alternative Arginine Utilization Pathways in Bacteria: Biochemical and Functional Perspectives, Catalytic Diversification of the Arginase Fold: Structural and Mechanistic Insights from DcsB, Beyond Arginase: Structural Biology of the Extended Guanidinase Family, Plant Arginase: Structural and Functional Roles in Nitrogen Metabolism, and much more.
Puntos claves
Puntos claves
- Provides comprehensive coverage of arginase from structure and mechanism to therapeutic targeting
- Includes methodology-driven chapters that emphasize experimental design, reproducibility, and analytical approaches
- Integrates academic and industry perspectives on inhibitor development, antibody-based and protein-based strategies, and translational applications
De interès para
De interès para
Índice
Índice
1. Expression, Purification, and Structural Characterization of Human and Leishmania Arginase with Comparative Analysis of Structures from the Protein Data Bank
E. D'Antonio, Luigi Franklin Di Costanzo and David W. Christianson
2. Synthesis of First-Generation Arginase Inhibitors
F. Sodano
3. Computational and Structure-Guided Design of Arginase Inhibitors
M.A. Fumagalli
4. Metal Substitution in Arginase: Experimental Approaches to Study Metal-Dependent Catalysis
George Georgiou
Section II: Advanced Therapeutic Strategies and Analytical Methods for Arginase
5. Therapeutic Arginase Formulations: PEGylation, Stability, and Advanced Delivery Systems
L. Sloan and M. Rudebeck
6. Dual ARG1/ARG2 Inhibition: Experimental and Structural Strategies for Inhibitor Design and Optimization
M. Grzybowski
7. Synthesis of Second-Generation Arginase Inhibitors
F. Sodano
8. Cryo-EM Workflow and Structural Characterization of Arginase–Antibody Complexes
G. Scapin
9. Development and Validation of an Electrochemical Assay for Arginase Activity
A. Miglione, Luigi Franklin Di Costanzo and Stefano Cinti
Section III: Clinical, Metabolic, and Translational Aspects of Arginase
10. Experimental and Structural Analysis of H. pylori Arginase in Gastric Immune Evasion
A.K. Sau
11. Agmatinase and Alternative Arginine Utilization Pathways in Bacteria: Biochemical and Functional Perspectives
J. French
12. Arginase Deficiency and Urea Cycle Disorders: Clinical and Metabolic Management
N. Brunetti-Pierri
13. Inborn Errors of Metabolism and Arginine Pathways: Experimental and Clinical Approaches
A. Schulze
14. Arginine Metabolism and Protein Balance: Experimental Approaches in Human Physiology
M. P. K. J. Engelen
15. Arginase in Endothelial Dysfunction and Vascular Disease: Experimental and Translational Approaches
W. Caldwell and R. Caldwell
16. Arginase in Cardiovascular and Vascular Inflammatory Disease
W. Durante
Section IV: Evolutionary and Functional Diversity of Arginase and Related Enzymes
17. Catalytic Diversification of the Arginase Fold: Structural and Mechanistic Insights from DcsB
Y. Matoba and Kosuke Oda
18. Beyond Arginase: Structural Biology of the Extended Guanidinase Family
G. Mlynek, S. V. Kundapura and D. Carugo
19. Plant Arginase: Structural and Functional Roles in Nitrogen Metabolism
B. Sekula
Detalles del producto
Detalles del producto
- Edición: 1
- Última edición
- Volumen: 740
- Publicado: 8 de diciembre de 2026
- Idioma: Inglés
Sobre el editor
Sobre el editor
LC
Luigi Di Costanzo
Luigi F. Di Costanzo is a structural chemist and biochemist specializing in structural biology, enzymology, and protein science, with a focus on metalloenzymes and structure-based approaches to enzyme function and inhibition. He earned his Ph.D. in Chemistry from the Università degli Studi di Trieste (Italy) and completed postdoctoral training at the University of Pennsylvania, where he contributed to foundational studies on enzyme structure and inhibitor design.
His research centers on the three-dimensional structure and mechanism of enzymes, with extensive experience in macromolecular crystallography, including structure determination, refinement, and analysis of protein–ligand complexes. His work integrates structural, biochemical, and biophysical approaches to understand enzyme function and guide therapeutic targeting.
In addition to his research, he served for over a decade as a biocurator with the Worldwide Protein Data Bank (wwPDB) at the RCSB PDB (Rutgers University), contributing to the validation and annotation of more than 7,500 macromolecular structures. This work supported the integrity and reproducibility of structural biology data used by the global scientific community, including structural datasets that have contributed to the development of machine learning approaches to protein structure prediction, such as AlphaFold.
His broader expertise includes multiscale structural characterization through complementary imaging and analytical techniques, including X-ray micro–computed tomography (µCT), linking atomic-level structure to complex biological systems, as well as contributions to community data resources, including the Protein Design Archive. As editor of the Methods in Enzymology volume “Arginase: Structure, Function, and Therapeutic Targeting,” he brings together structural, metabolic, and translational perspectives to advance the field.