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Elsevier

Laboratory Methods in Enzymology: RNA

  • 1 Edición, Volumen 530 - 11 de septiembre de 2013
  • Última edición
  • Autor: Jon Lorsch
  • Idioma: Inglés

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-… Leer más

Descripción

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Puntos claves

  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on RNA

De interès para

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Índice

Computational Prediction of RNA Secondary Structure

Reverse-transcription PCR (RT-PCR)

Northern blotting

Explanatory Chapter: Nuclease Protection Assays

In Vitro Transcription from Plasmid or PCR-amplified DNA

Measurement of In Vivo RNA Synthesis Rates

Method for measuring mRNA decay rate in Saccharomyces cerevisiae

Analysis of polysomes from bacteria

Polysome Profile Analysis – Yeast

Polysome analysis of mammalian cells

Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae

Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates

Circularized RT-PCR (cRT-PCR): analysis of the 5′ ends, 3′; ends, and poly(A) tails of RNA

RNA Radiolabeling

Fluorescently Labeling Synthetic RNAs

Analysis of RNA by analytical polyacrylamide gel electrophoresis

RNA purification by preparative polyacrylamide gel electrophoresis

Explanatory Chapter: Nucleic Acid Concentration Determination

RNA purification – precipitation methods

Reverse Transcriptase Dideoxy Sequencing of RNA

RNA Structure Experimental Analysis – Chemical Modification

Structural Analysis of RNA Backbone Using In-Line Probing

Detalles del producto

  • Edición: 1
  • Última edición
  • Volumen: 530
  • Publicado: 11 de septiembre de 2013
  • Idioma: Inglés

Sobre el autor

JL

Jon Lorsch

Afiliaciones y experiencia
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA

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