Metal-Containing Enzymes

Preface
One: Type-3 Copper Proteins: Recent Advances on Polyphenol Oxidases
Abstract

1 Introduction

2 Primary Structure and Molecular Weights of PPOs

3 Oxo Complex

4 X-ray Crystallographic Structural Data of PPOs and Hemocyanins

5 Conclusions and Outlook
Acknowledgments
Two: Biophysical Studies of Matrix Metalloproteinase/Triple-Helix Complexes
Abstract

1 MMPs and Collagen Hydrolysis

2 Structures of Full-Length, Collagenolytic MMPs in Solution and in the Solid State

3 Structural Evaluation of MMP Interactions with Collagen

4 Mechanism of Collagenolysis

5 Heterogeneity in MMP Structures
Acknowledgment
Three: Catalytic Mechanisms of Metallohydrolases Containing Two Metal Ions
Abstract

1 Introduction

2 Metallo-β-Lactamases, Major Culprits in the Emergence of Antibiotic Resistance

3 Methionine Aminopeptidase, a Target for Novel Anticancer Drugs

4 Glycerophosphodiesterase, a Very Promiscuous Potential Bioremediator

5 PAP, an Alternative Target to Treat Osteoporosis

6 Conclusions and Outlook: Agmatinase, an Emerging Target for Biotechnological Applications
Acknowledgments
Four: Applications of Quantum Mechanical/Molecular Mechanical Methods to the Chemical Insertion Step of DNA and RNA Polymerization
Abstract

1 Introduction

2 Methods for Describing Reactive Pathways

3 DNA Polymerase β

4 Application of QM/MM to Systems Similar to Pol β

5 RNA Polymerase

6 Thoughts for Future QM/MM Simulations on Ternary Substrate Complexes of Nucleic Acid Polymerases

7 Conclusions
Acknowledgments
Five: Monitoring the Biomolecular Interactions and the Activity of Zn-Containing Enzymes Involved in Conformational Diseases: Experimental Methods for Therapeutic Purposes
Abstract

1 Zn-Metalloproteases and Conformational Diseases

2 Analytical Techniques Used to Study ZnMPs-Substrate/Inhibitors Interactions

3 Conclusions and Future Perspectives
Author Index
Subject Index