Molecular Breeding and Genetics of Applied Microorganisms

ContentsContributorsPreface Introduction1. Species Barriers to the Maintenance and Expression of Foreign DNA 1.1 Barriers to the Genetic Establishment and Expression of Foreign DNA 1.2 Selective Disadvantage of Hosts Harboring Recombinant Molecules 2. Regulation of Antibiotics Production in Microorganisms 2.1 Regulation of the Initiation of Secondary Metabolism 2.2 Characteristics of Enzymes Involved in Secondary Metabolism 2.3 Quantitative Regulation of Antibiotics Production 3. Plasmid Involvement in Antibiotics Production 3.1 The Nature of Secondary Metabolites 3.2 Evidence of Plasmid Involvement 3.3 Plasmid Involvement in Chloramphenicol Production 3.4 Plasmid Involvement in Aureothricin Production 3.5 Plasmid DNA in Streptomycetes 3.6 Plasmid Function in Antibiotics Production 4. Degradative Plasmids: Aspects of Microbial Evolution 4.1 Introduction 4.2 Physiology of Degradative Plasmids 4.3 Phenotype of Degradative Plasmids 4.4 Handling of Degradative Plasmids 4.5 Adaptation and Evolution 4.6 Closing Remarks 5. Selection of Genes with Antibodies against the Gene Products 5.1 Purification of Calf Rennin mRNA 5.2 Bovine Corticotropin-β-Lipotropin Precursor 6. Enzymatic Synthesis of Polynucleotides of Defined Sequence 6.1 Polynucleotide Phosphorylase 6.2 RNA Ligase 7. Protoplast Fusion 7.1 Brevibacterium 7.2 Streptomyces 7.3 Saccharomyces 7.4 Aspergillus 8. Cloning with Recombinant DNA 8.1 Bacillus subtilis 8.2 Development of Molecular Cloning Systems in Pseudomonas 8.3 Highly Thermophilic Bacteria 8.4 Cloning of Streptomyces DNA in Escherichia colt 9. New Genetic Approaches to Industrial Microorganisms 9.1 Amino Acid-Producing Strains 9.2 Fermentative Production of "Magic Spots" Subject Index Index of Microorganisms